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TechInfo
 
      Worksheet No*

      Title
E1 General procedure for indirect sandwich ELISA
E2 General procedure for indirect sandwich ELISA using Streptavidin- Biotin detection
E3 General procedure for direct ELISA
E4 Quantitation of human cytokines by ELISA using Biozol matched pair reagents
FC 1 Direct immunofluorescence staining for flow cytometry
FC 2 Indirect immunofluorescence staining for flow cytometry
FC 3 Direct immunofluorescence staining of immunoglobulin
light chains on B lymphocytes in whole blood by flow cytometry
FC 4 Direct staining of intracellular antigens by flow cytometry
FC 5 Direct staining of intracellular antigens by flow cytometry - methanol modification
FC 6 Direct staining of intracellular cytokines by flow cytometry
FC 7 Protocol for intracellular staining with Leucoperm (FIX &PERM)
FC 8 Whole blood protocol for analysis of intracellular cytokines
FC 9 Digitonin permeabilisation of cells for flow cytometry
FC 10 Direct staining of intracellular antigens by flow cytometry – the paraformaldehyde/saponin method
FC 11 Use of the Biozol dendritic cell exclusion cocktail for identification and
phenotyping of peripheral blood dendritic cells
IF 1 Direct immunofluorescence staining for fluorescence microscopy
IF 2 Staining of DNA-synthesizing cells with BrdU-FITC
IH 1 Indirect immunostaining of frozen tissue sections
IH 2 Streptavidin - Biotin immunostaining of frozen tissue sections
IH 3 PAP / APAAP immunostaining of frozen tissue sections
IH 4 Indirect immunostaining of paraffin embedded tissue sections
IH 5 Streptavidin - Biotin immunostaining of paraffin embedded tissue sections
IH 6 PAP / APAAP immunostaining of paraffin embedded tissue sections
IH 7 Antigen retrieval techniques for use with formalin-fixed paraffin-embedded sections
IH 8 Fixative recipes
IP 1 Immunoprecipitation protocol
WB 1 Western blotting
   
*E: ELISA FC: flow cytometry IF: immunofluorescence microscopy IH: immunohistochemistry
IP: immunoprecipitation WB: western blotting