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Worksheet No*	Title
E1	General procedure for indirect sandwich ELISA
E2	General procedure for indirect sandwich ELISA using Streptavidin- Biotin detection
E3	General procedure for direct ELISA
E4	Quantitation of human cytokines by ELISA using Biozol matched pair reagents
FC 1	Direct immunofluorescence staining for flow cytometry
FC 2	Indirect immunofluorescence staining for flow cytometry
FC 3	Direct immunofluorescence staining of immunoglobulin light chains on B lymphocytes in whole blood by flow cytometry
FC 4	Direct staining of intracellular antigens by flow cytometry
FC 5	Direct staining of intracellular antigens by flow cytometry - methanol modification
FC 6	Direct staining of intracellular cytokines by flow cytometry
FC 7	Protocol for intracellular staining with Leucoperm (FIX &PERM)
FC 8	Whole blood protocol for analysis of intracellular cytokines
FC 9	Digitonin permeabilisation of cells for flow cytometry
FC 10	Direct staining of intracellular antigens by flow cytometry – the paraformaldehyde/saponin method
FC 11	Use of the Biozol dendritic cell exclusion cocktail for identification and phenotyping of peripheral blood dendritic cells
IF 1	Direct immunofluorescence staining for fluorescence microscopy
IF 2	Staining of DNA-synthesizing cells with BrdU-FITC
IH 1	Indirect immunostaining of frozen tissue sections
IH 2	Streptavidin - Biotin immunostaining of frozen tissue sections
IH 3	PAP / APAAP immunostaining of frozen tissue sections
IH 4	Indirect immunostaining of paraffin embedded tissue sections
IH 5	Streptavidin - Biotin immunostaining of paraffin embedded tissue sections
IH 6	PAP / APAAP immunostaining of paraffin embedded tissue sections
IH 7	Antigen retrieval techniques for use with formalin-fixed paraffin-embedded sections
IH 8	Fixative recipes
IP 1	Immunoprecipitation protocol
WB 1	Western blotting
*E: ELISA FC: flow cytometry IF: immunofluorescence microscopy IH: immunohistochemistry IP: immunoprecipitation WB: western blotting

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